FSS Phyto-Biotics Perilla®

INCI: Perilla Frutescens Extract & Lactobacillus Ferment

Use Levels: 1.0% - 10.0%

Solubility: Water Soluble

Appearance: Clear to Slightly Hazy Liquid
pH (Direct): 3.5 - 5.5

The demand for stem cell technologies has grown exponentially in the last decade. Trending specifically in the cosmetic market, current stem cell technologies utilize Meristematic cells, non-differentiating plant stem cells from simple cell extracts with no specific activity. To go beyond the current offerings on the market, it was chosen to use biotic stress, embodied by the sustainable practice of co-culturing of plant stem cells with Leuconostoc sp., to promote the formation of secondary metabolites. This novel approach induces cellular differentiation and provides formulators with the ability to finely customize formulations, specify activity and skin benefits while offering brand distinction. FSS Phyto-Biotics Perilla capitalizes on specific activity of the phenolic metabolite, Rosmarinic Acid, produced by Perillia frutescens, or Chinese Basil, to provide the cosmetic market with a plant- derived, stem cell ingredient designed to impart antioxidant, protectant, antimicrobial and anti-aging benefits while enhancing cellular metabolism and improving radical stability.

BENEFITS

The destruction of plants continues to pose a major threat to the plant species. Commercially, the medicinal and cosmetic use of plant secondary metabolites involves isolating these organic compounds through solvent extraction of the naturally grown, whole plant. With the possibility of extinction at any given time, the development of alternative and complimentary methods to whole plant extract for the production of these organic compounds, particularly for commercial purposes, is an issue of considerable socioeconomic importance. By using cell cultures, industries can grow numerous plants, creating a sustainable source of plant extracts without disrupting the environment.

As previously mentioned, using solvent extraction from a naturally grown, a whole plant is environmentally damaging. Using biotic stress, specifically pathogenic stress via Leuconostoc sp., the formulators created FSS Phyto- Biotics Perilla by inducing the production of the phenolic compound, Rosmarinic Acid, to create a plant stem cell product ideal for anti-aging, antimicrobial and soothing cosmetic applications.

EFFICACY
To demonstrate the improvements in Rosmarinic Acid content in Perilla frutescens co-cultured with Pseudomonas sp., an efficacy assay was performed comparing it to un-inoculated controls. Genetically uniform, shoot-based clonal lines of Perilla frutescens were isolated and co-cultured in 1 mg/ 1 benzylaminopurine in standard Murashige and Skoog medium with 3.0% sucrose and inoculated with Pseudomonas sp. for thirty (30) days. The controls consisted of seven (7), un-inoculated clonal lines of Perilla frutescens. After thirty (30) days, Rosmarinc Acid was extracted from 50 mg of the plant tissues with 2ml of 50% (v/v) methanol for one (1) hour at 55°C. After cooling to room temperature, 1 ml of extract was diluted by adding 3 ml of 50.0% (v/v) methanol. The absorbance was measured at 333 nm with a Spectronic.RTM.Genesys.TIM.5 spectrophotometer. The improvements in Rosmarinic Acid content in the Perilla frutescens herbal plants co-cultured with Pseudomonas sp. in comparison to the control are clearly indicated in the following graph:

To demonstrate the protective benefits of Rosmarinic Acid, a test was contucted on H2O2. To examine the effects of Rosmarinic Acid on H2O2 the cultured solutions containing Rosmarinic Acid, at each concentration from 100 to 130 nM, were incubated for two (2) hours.

An Oxygen Radical Absorbance Capacity Assay was conducted in-house to measure the antioxidant activity of FSS Phyto- Biotics Perilla. FSS Phyto- Biotics Perilla co-cultured with Leuconostoc sp. standardized for Rosmarinic Acid was compared with a Trolox Control (Vitamin E analogue and antioxidant standard for free radical absorption). The results indicated that FSS Phyto- Biotics Perilla exhibited strong antioxidant activity at 0.1%. As demonstrated in the graph, FSS Phyto- Biotics Perilla has antioxidant activity comparable to that of Trolox. Due to it’s potent antioxidant activity, FSS Phyto-Biotics Perilla can be used in cosmetic applications to impart anti-aging benefits compliments of the product’s potent antioxidant properties.

Storage: Protected from direct light and humidity at a temperature of 50°-77°F (10°-25°C) 

Shelf life: 12 months, properly stored, in sealed container. 

This product should be added to a formulation at the recommended usage rate.